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Gamborg B5 | Medium Vitamins & Basal Salts
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Gamborg B5 Medium with Vitamins & Basal Salts
Gamborg B5 is a widely recognized plant tissue culture medium, originally developed in 1968 for the cultivation of soybean root cell suspensions. Its composition features reduced total salt concentration compared to classical formulations, making it particularly suitable for cell cultures, callus induction, and protoplast work. The medium includes all essential macro- and micronutrients, along with key vitamins such as myo-inositol, thiamine HCl, and nicotinic acid, providing a well-balanced environment for consistent in vitro development.
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Gamborg B5 Medium is a widely used plant tissue culture medium, originally formulated by O.L. Gamborg in 1968 for soybean cell suspension cultures. It contains a balanced mix of inorganic salts, micronutrients, and vitamins to support plant cell growth, callus induction, and maintenance of cell cultures.
Compared to Murashige & Skoog (MS) medium, Gamborg B5 has:
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Lower ammonium content (reduced stress for certain plant cells)
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Higher potassium nitrate as the primary nitrogen source
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Increased thiamine (Vitamin B1) concentration
This formulation is particularly suitable for:
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Cell suspension cultures
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Callus induction and proliferation
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Protoplast culture
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Early-stage plant regeneration experiments
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Gamborg B5 Medium with Vitamins & Basal Salts
Gamborg B5 is a widely recognized plant tissue culture medium, originally developed in 1968 for the cultivation of soybean root cell suspensions. Its composition features reduced total salt concentration compared to classical formulations, making it particularly suitable for cell cultures, callus induction, and protoplast work. The medium includes all essential macro- and micronutrients, along with key vitamins such as myo-inositol, thiamine HCl, and nicotinic acid, providing a well-balanced environment for consistent in vitro development.