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Woody Plant Medium (WPM) with Vitamins & Basal Salts
Woody Plant Medium (WPM) is a specialized formulation developed in 1980 to support the growth and micropropagation of woody species. Compared to classical plant tissue culture media, WPM contains lower levels of total salts and nitrogen, making it especially suitable for trees, shrubs, and other woody plants that are sensitive to higher ionic concentrations. The medium provides a balanced mix of macro- and micronutrients along with essential vitamins such as thiamine HCl, pyridoxine HCl, and nicotinic acid, creating an optimized environment for shoot initiation, callus culture, and in vitro rooting of a wide range of woody species.
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Woody Plant Medium (WPM) is a specialized plant tissue culture medium, originally formulated by Lloyd and McCown in 1980 for the propagation of woody species such as trees and shrubs. It contains a carefully balanced composition of inorganic salts, micronutrients, and vitamins to support the growth and development of plants that are sensitive to high salt concentrations.
Compared to Murashige & Skoog (MS) medium, WPM has:
Lower total salt concentration (better suited for woody species)
Reduced nitrogen levels (minimizing stress for salt-sensitive plants)
Adjusted calcium and magnesium content (supporting robust shoot and root development)
This formulation is particularly suitable for:
Micropropagation of woody plants (trees, shrubs, fruit crops)
In vitro rooting of sensitive plant species
Callus culture and shoot initiation in woody perennials
Long-term maintenance of woody plant tissues